AGAR VRBD (VIOLET RED BILE DEXTROSE) MERCK 500G

Marca: MERCK

Referência: 1102750500

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Descrição Geral

VRBD AGAR (VIOLET RED BILE DEXTROSE)SEG HARM EP/USP/JP MERCK 500GR

Mode of Action
Crystal violet and bile salts inhibit the accompanying bacterial flora. Degradation of glucose is accompanied by production of acid, which is indicated by a colour change to red and by zones of precipitated bile acids surrounding the colonies. All Enterobacteriaceae are detected as they all degrade glucose to acid. The
culture medium is not, however, absolutely specific for these organisms as some other accompanying bacteria (e.g. Aeromonas) also show these reactions.

Typical Composition (g/litre)
Peptone from gelatine 7.0;
yeast extract 3.0;
sodium chloride 5.0;
D(+)glucose 10.0;
bile salt mixture 1.5;
neutral red 0.03;
crystal violet 0.002;
agar-agar 13.0

Preparation
Suspend 39.5 g in 1 litre of demin. water and heat to boiling with frequent stirring until completely dissolved. Afterwards do not boil more than 2 minutes.
Do not autoclave! Do not overheat!
pH: 7.3 ± 0.2 at 25 °C.
The prepared medium is clear and dark red.
Incubation: for 24 hours at 35 °C aerobically.
The identity of suspected Enterobacteriaceae colonies should be confirmed by further tests.

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Itens Inclusos

Frasco com 500gr

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