CALDO LAURIL SULFATO COM MUG 500GR, MERCK

FLUOROCULT LAURYL SULFATE BROTH (LST MUG MEDIUM) MERCK P/ MICROBIOLOGIA
RENDIMENTO 36,5g/l (CALDO LAURIL SULFATO C/MUG)
EMBALAGEM 500GR

The medium complies with the German-DIN-Norm 10183 for the examination of milk, with the regulations acc. to par.35 LMBG (01.00/54) for the examination of food, and acc. to ISO/DIS 11886 - 2.2 (1994) for milk and milk products. Furthermore to the German Badegewässerverordnung (regulations for bathing water) 76/1604 EWG (1995).

Mode of Action
The lauryl sulfate largely inhibits the growth of undesirable microbial flora. The presence of E. coli is indicated by fluorescence under a long wavelength UV lamp. A positive indole reaction and gas formation due to fermentation of lactose confirm the results. SCHINDLER (1991) recommended the use of this medium in the
quality control of bathing water.

Typical Composition (g/litre)
Tryptose 20.0;
lactose 5.0;
sodium chloride 5.0;
sodium lauryl sulfate 0.1;
di-potassium hydrogen phosphate 2.75;
potassuim dihydrogen phosphate 2.75;
L-tryptophan 1.0;
4-methylumbelliferyl-b-D-glucuronide 0.1.

Preparation
Suspend 36.5 g/litre, fill in test tubes fitted with DURHAM tubes; autoclave (15 min at 121 °C).
pH: 6.8 ± 0.2 at 25 °C.
The prepared broth is clear and yellowish-brown.

Imagem meramente ilustrativa.