BCYE Agar - Legionella Isolation Medium 500g - Acumedia

Description
n 1977, McDade et al. identified Legionella pneumophila as the causative agent of Legionnaires’ disease, a multisystem disease manifested primarily by pneumonia. In 1978 a new medium, F-G Agar, resulted in improved growth of L. pneumophila, a very fastidious organism. Freely et al. modified F-C Agar by substituting yeast extract as a vitamin source and replacing starch with activated charcoal, producing Charcoal Yeast Extract (CYE) Agar. In 1980, Pasculle et al. reported that CYE Agar could be improved by the addition of ACES (N-2-acetamido-2-aminoethane sulfonic acid) buffer. One year later, Edelstein further increased the sensitivity of the medium by adding the potassium salt of alphaketoglutaric acid.

Typical Formulation
Yeast Extract 10.0 g/L
Charcoal 2.0 g/L
Ferric Pyrophosphate 0.25 g/L
ACES Buffer 10.0 g/L
Potassium Carbonate 2.3g/L
Agar 14.0 g/L
Final pH: 6.9 ± 0.1 at 25C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplements ( NOT INCLUDED)
NCM4007 GVPC Selective Supplement
NCM4006 BCYE Growth Supplement
NCM4005 BCYE Growth Supplement (without L-Cysteine)